Updated July 2006
Unit of Clinical Immunology
Department of Medicine
Hadassah University Hospital
Ein-Karem, Jerusalem, Israel.
Phone: 02-6777817
Fax: 02-6437775
Date of Birth: December 8, 1942
Place of Birth: Kibbutz Ashdot Ya'acov, Israel
Marital Status: Married (1968), 3 children
1960 - Graduated from Hadera Secondary School
1960 – 1963 Military Service
1964 – 1967 Studies at the Hebrew University of Jerusalem. Subjects: Chemistry and Biochemistry
1967 - B.Sc.
1968 – 1969 M.Sc. studies, Department of Biological Chemistry, the Hebrew University of Jerusalem. M.Sc. Thesis: "Studies on the chemical synthesis of alanine-containing peptidyl-tRNA" under the supervision of Dr. Y. Lapidot.
1969 - M.Sc. Degree Assistant, Department of Biological Chemistry, the Hebrew University of Jerusalem. Ph.D. Thesis: "Chemical and Biological Properties of Peptidyl-tRNA" under the supervision of Dr. Y. Lapidot and close collaboration with Dr. C.R. Cantor of Columbia University.
1973 Instructor, Department of Biological Chemistry, the Hebrew University of Jerusalem.
1974 - Thesis submitted and approved by the Senate of the Hebrew University of Jerusalem.
1975 – 1977 Visiting Fellow at the Laboratory of Chemical Biology, NIAMDD, National Institutes of Health. Chief: Dr. Christian B. Anfinsen. Section Head: Dr. Alan N. Schechter. Close collaboration with Dr. Alfred D. Steinberg. Training: Protein Chemistry, Immunochemistry.
1977 – 1978 Visiting Associate at the Laboratory of Microbial Immunity. NIAID, National Institutes of Health. Chief: Dr. Richard Asofsky.
1978 - Investigator, Clinical Immunology Unit, Department of Medicine A, Hadassah University Hospital, Jerusalem, Israel.
1979 - Lecturer in Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
1982 – 1986 Senior Lecturer in Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
1986 – 1993 Associate Professor of Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
1990 – 1991 Visiting Scientist at the Laboratory of Immunology, NIAID, National Institutes of Health. Chief: Dr. William E. Paul. Section Head: Dr. Ethan M. Shevach.
1983 Professor of Immunology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
1998 Visiting Scientist at the Laboratory of Immunology, National Institutes of Health. Chief: Dr. William E. Paul. Section Head: Dr. David H. Margulies.
List of Publications
1. Lapidot, Y., de Groot, N., Rappoport, S., and Eilat, D. Peptidyl-tRNA VIII. The chemical synthesis of glycine and alanine containing oligopeptidyl-tRNA. Biochem. Biophys. Acta. 190:304-311, 1969.
2. Lapidot, Y., Eilat, D., Rappoport, S., and de Groot, N. Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA. Biochem. Biophys. Res. Commun. 38:559-563, 1970.
3. Oen, H., Pellegrini, M., Eilat, D., and Cantor, C.R. Identification of 50 S protein at the peptidyl-tRNA binding site of Escherichia coli ribosomes. Proc. Nat. Acad. Sci. U.S.A. 70:2799-2803, 1973.
4. Pellegrini, M., Oen, H., Eilat, D., and Cantor, C.R. The mechanism of covalent reaction of BrAcPhe-tRNA with the peptidyl-tRNA binding site of E. coli ribosome. J. Mol. Biol. 88:809-829, 1974.
5. Eilat, D., Pellegrini, M., Oen, H., Lapidot, Y., de Groot, N., and Cantor, C.R. A chemical mapping technique for exploring the location of proteins along the ribosome-bound peptide chain. J.Mol. Biol. 88:831-840, 1974.
6. Eilat, D., Pellegrini, M., Oen, H., Lapidot, Y., de Groot, N., and Cantor, C.R. Affinity labelling the acceptor site of the peptidyl-transferase center of the Escherichia coli ribosome. Nature. 250:514-516, 1974.
7. Eilat, D., Ziv, E., and Lapidot, Y. Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns. Nucleic Acid Research. 2:2237-2250, 1976.
8. Di Natale, P., and Eilat, D. Patterns of E. coli leucyl-tRNA isoacceptors following bacteriophage MS2 infection. Nucleic Acid Research. 3:917-930, 1976.
9. Eilat, D., Steinberg, A.D., and Schechter, A.N. Antibodies to native tRNA in NZB/NZW mice. Nature. 259:141-143, 1976.
10. Eilat, D., Di Natale, P., Steinberg, A.D., and Schechter, A.N. Propertis of tRNA specific antibodies from NZB/NZW mice. J. Immunol. 118:1016-1027, 1977.
11. Eilat, D., Steinberg, A.D., and Schechter, A.N. The reaction of SLE antibodies with native, single stranded RNA: Radioassay and binding specificities. J. Immunol. 120:550-557, 1978.
12. Eilat, D., Reeves, J.P., Steinberg, A.D., and Schechter, A.N. Measurement of antigen-specific IgG autoantibody production in vitro. J. Immunol. 1211:310-314, 1978.
13. Eilat, D., and Chaiken, I.M. Expression of multivalency in the affinity chromatography of antibodies. Biochemistry. 18:790-794, 1979.
14. Chaiken, I.M., Eilat, D., and McCormick, W.M. Appendix: Derivation and evaluation of equations for independent bivalent interacting systems in quantitative affinity chromatography. Biochemistry. 18:794-795, 1979.
15. Eilat, D., Asofsky, R., and Laskov, R. A hybridoma from an autoimmune NZB/NZW mouse producing monoclonal antibody to ribosomal-RNA. J. Immunol. 124:766-768, 1980.
16. Eilat, D., Asofsky, R., and Laskov, R. Fine antigenic specificity of RNA-binding monoclonal antibody from autoimmune NZB/NZW mouse. Proceedings of the 28th Annual Colloquium on Protides of the Biological Fluids (H. Peeters, ed). Academic Press, pp. 583-586, 1980.
17. Tack, B.F., Dean, J., Eilat, D., Lorenz, P.E., and Schechter, A.N. Tririum labeling of proteins to high specific activity by reductive methylation. J. Biol. Chem. 255:8842-8847, 1980.
18. Eilat, D., Ben Sasson, S.A., and Laskov, R. A ribonucleic acid specific antibody produced during autoimmune disease: Evidence for nucleotide sequence specificity. Eur. J. Immunol. 10:841-849, 1980.
19. Eilat, D., and Laskov, R. Production of anti-RNA antibody by hybridoma cells: Purification from mixed immunglobulin products. Molec. Immunol. 18:589-595, 1981.
20. Eilat, D., Fischel, R., and Zlotnick, A. Albumin-immunoglobulin complexes in human serum: classification and immunochemical analysis. Scand. J. Immunol. 14:77-87, 1981.
21.Eilat, D., and Lotan, C. The reaction of human SLE antibodies with native, single stranded RNA: Analysis of nucleotide sequence specificities and correlation with Sm and nRNP activities. Clin. Exp. Immunol. 49:283-289, 1982.
22. Zlotnick, A., Ben-Chetrit, E., and Eilat, D. Bence-Jones cryoprotein in a patient with multiple myeloma. Harefuah. 53:91-93, 1982. (In Hebrew).
23. Eilat, D., Hochberg, M., Fischel, R., and Laskov, R. Antibodies to RNA from autoimmune NZB/NZW mice recognize a similar antigenic determinant and show a large idiotypic diversity. Proc. Natl. Acad. Sci. U.S.A. 79:3818-3822, 1982.
24. Eilat, D. Monoclonal autoantibodies: An approach to studying autoimmune disease. A review. Molec. Immunol. 19:943-955, 1982.
25. Laskov, R., Muller, S., Hochberg, M., Giloh, H., Van Regenmortel, M.H.V., and Eilat, D. Monoclonal autoantibodies to histones from autoimmune NZB/NZW mice. Eur. J. Immunol. 14:74-81, 1984.
26. Eilat, D., Hochberg, M., Pumphrey, J., and Rudikoff, S. Monoclonal antibodies to DNA and RNA from NZB/NZW mice: Antigenic specificities and NH2 terminal amino acid sequences. J. Immunol. 133:489-494, 1984.
27. Eilat, D. Immunotoxins - a new approach for selective immunotherapy. A review. Harefuah. 56:315-318, 1984. (In Hebrew).
28. Eilat, D., Fischel, R., and Zlotnick, A. A central anti-DNA idiotype in human and murine systemic lupus erythematosus. Eur. J. Immunol. 15:368-375, 1985.
29. Eilat, D., Fischel, R., and Zlotnick, A. Autoantibodies to anti-DNA with anti-allotypic and anti-idiotypic specificities in NZB/NZW mice. Eur. J. Immunol. 15:375-381, 1985.
30. Ben-Chetrit, E., Dunsky, E., Wollner, S., and Eilat, D. In vivo clearance and tissue uptake of an anti-DNA monoclonal antibody and its complexes with DNA. Clin. Exp. Immunol. 60:159-168, 1985.
31. Eilat, D. Cross-reactions of anti-DNA antibodies and the central dogma of lupus nephritis. Immunology Today. 6:123-127, 1985.
32. Shouval, D., Eilat, D., Carlson, R.I. Adler, R., Livni, N., and Wands, J.R. Human hepatoma associated cell surface antigen: identification and characterization by means of monoclonal antibodies. Hepatology 5:347-356, 1985.
33. Eilat, D., Ben-Chetrit, E., Fischel, R., Zlotnick, A.Y., and Hochberg, M. Immunochemical and biological properties of anti-nucleic acid antibodies in SLE. Proceedings of the 33rd Annual Colloquium on Protides of the Biological Fluids (H. Peeters, editor), Pergamon Press, pp. 228-231, 1985.
34. Eilat, D. Protein and gene structure of autoantibodies in the study of autoimmune disease. Acta Haematologica, 76:101-106, 1986.
35. Eilat, D. Anti-DNA antibodies: problems in their study and interpretation. A review. Clin. Exp. Immunol. 65:212-222, 1986.
36. Eilat, D., Zlotnick, A.Y., and Fischel, R. Evaluation of the cross-reaction between anti-DNA and anti-cardiolipin antibodies in SLE sera and experimental animals. Clin. Exp. Immunol. 65:269-278, 1986.
37. Eilat, D. Genes, idiotypes and antigenic specificity of anti-nucleic acid antibodies in autoimmune disease. Seminaire International D'Immunopathologie Articulaire. Montpellier. 1987, pp. 255-264.
38. Eilat, D, Webster, D., and Rees, A.R. Variable region sequences of anti-DNA and anti-RNA autoantibodies from NZB/NZW F1 mice. J. Immunol. 141:1745-1753, 1988.
39. Ben-Chetrit, E., Eilat, D., and Ben-Sasson, S.A. Specific inhibition of the DNA-anti-DNA immune reaction by low molecular weight anionic compounds. Immunology 65:479-485, 1988.
40. Eilat, D. The measurement of anti-DNA activity in the sera of patients with systemic lupus erythematosus: Theoretical and practical considerations. Autoimmunity, 3:299-306, 1989.
41. Eilat, D., Hochberg, M., Tron, F., Jacob, L,. and Bach, J.-F. The VH gene sequences of anti-DNA antibodies in two different strains of lupus-prone mice are highly related. Eur. J. Immunol. 19:1241-1246, 1989.
42. Harats, N., Fischel, R., Rubinow, A., and Eilat, D. IgG rheumatoid factor in purified IgG fractions and whole sera in rheumatoid arthritis. Br. J. Rheumatol. 28:227-232, 1989.
43. Raz, E., Brezis, M., Rosenmann, E. and Eilat, D. Anti-DNA antibodies bind directly to renal antigens and induce kidney dysfunction in the isolated perfused rat kidney. J. Immunol. 142:3076-3082, 1989.
44. Eilat, D. The role of germline gene expression and somatic mutation in the generation of autoantibodies to DNA. Molecular Immunol. 27:203-210, 1990.
45. Eilat, D. Immunoglobulin and T cell receptor genes in SLE. In: Immunogenetics of Autoimmune Disease (Ed. Farid, N.R.), Vol. II pp 1-30, CRC Press, 1990.
46. Eilat, D. Origines Genetiques des Auto-anticorps Anti-ADN. Ann. Med. Intern. 141: 205-207, 1990.
47. Eilat, D., and Fischel, R. Recurrent utilization of genetic elements in variable regions of anti-nucleic acid antibodies from autoimmune mice. J. Immunol. 147:361-368, 1991.
48. Eilat, D., Kikuchi, G., Coligan, J.E., and Shevach, E.M. Secretion of a soluble, chimeric T cell receptor - immunoglobulin heterodimer. Proc. Natl. Acad. Sci. U.S.A. 89:6871-6875, 1992.
49. Fischel, R., and Eilat, D. The structure and binding properties of a monoclonal antiidiotypic autoantibody to anti-DNA with epibody activity. J. Immunol. 149:3089-3096, 1992.
50. Raz, E., Ben-Bassat, H., Davidi, T., Shlomai, Z., and Eilat, D. Cross-reactions of anti-DNA autoantibodies with cell surface proteins. Eur. J. Immunol. 23:383-390, 1993.
51. Staines, N.A., Ward, F.J., Denbury, A.N., Mitchiner, J., Hartley, O., Eilat, D., Isenberg, D.A., and Bansal, S. Primary sequence and location of the idiotopes of V-88, a DNA-binding monoclonal autoantibody, determined by idiotope scanning with synthetic peptides on pins. Immunology. 78:371-378, 1993.
Regan, J.R., Bruno, J.G., Chang, M.N., Sabatino, R., D'Alisa, R., Ben-Sasson, S.A., and Eilat, D. Mimicry of biological macromolecules by polyaromatic anionic compounds. J. Bioactive Compatible Polymers. 8:317-337, 1993.
53. Eilat, D., and Anderson, W.F. Structure-function correlates of autoantibodies to nucleic acids: Lessons from immunochemical, genetic and structural studies. A review. Molecular Immunology. 31:1377-1390, 1994.
54. Pewzner-Jung, Y., Simon, T., and Eilat D. Structural elements controlling anti-DNA antibody affinity and their relationship to anti-phosphorylcholine activity. J. Immunol. 156:3065-3073, 1996.
55. Sonoda, E., Pewzner-Jung, Y., Schwers, S., Taki, S., Jung, S., Eilat, D., and Rajewsky, K. B-cell development under the condition of allelic inclusion. Immunity. 6:225-233, 1997.
56. Ash-Lerner, A., Ginsberg-Strauss, M., Pewzner-Jung, Y., Marion, T.N., and Eilat, D. Expression of an anti-DNA associated VH gene in immunized and autoimmune mice. J. Immunol. 159:1508-1519, 1997.
57. Pewzner-Jung, Y., Friedmann, D., Sonoda, E., Jung, S., Rajewsky, K., and Eilat, D. B cell deletion, anergy and receptor editings in “knock-in” mice targeted with a germline-encoded or somatically mutated anti-DNA heavy chain. J. Immunol. 161:4634-4645, 1998.
58. Jovelin, F., Mostoslavsky, G., Amoura, Z., Chabre, H., Gilbert, D., Eilat, D., Bach, J.-F., and Koutouzov, S. Early anti-nucleosome autoantibodies from a single MRL +/+ mouse: Fine specificity, V gene structure and pathogenicity. Eur. J. Immunol. 28:3411-3422, 1998.
59. Friedmann, D., Yachimovich, N., Mostoslavsky, G., Pewzner-Jung, Y., Ben-Yehuda, A., Rajewsky, K., and Eilat, D. Production of high affinity autoantibodies in autoimmune NZB/NZW F1 mice targeted with an anti-DNA heavy chain. J. Immunol. 162:4406-4416, 1999.
60. Eilat, D., and Naparstek, Y. Anti-DNA autoantibodies: A puzzle of autoimmune phenomena. Immunol. Today. 20:339-342, 1999.
61. Natarajan, K., Boyd, L.F., Schuck, P., Yokoyama, W.M., Eilat, D., and Margulies, D.H. Interaction of the NK cell inhibitory receptor Ly49A with H-2Dd: Identification of a site distinct from the TCR site. Immunity 11:591-601, 1999
62. Mostoslavsky, G., Fischel, R., Yachimovich, N., Yarkoni, Y., Rosenmann, E., Monestier, M., Baniyash, M., and Eilat, D. Lupus anti-DNA autoantibodies cross-react with a glomerular structural protein: A case for tissue injury by molecular mimicry. Eur. J. Immunol. 31:1221-1227, 2001
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63. Yachimovich, N., Mostoslavsky, G., Yarkony, Y., Fischel, R., Verbovetski, I., and Eilat, D. The efficiency of B cell receptor editing is dependent on BCR L-chain rearrangement status. Eur. J. Immunol. 32:1164-1174, 2002
64. Yachimovich-Cohen, N., Fischel, R., Yarkony, Y., Bachar., N., and Eilat, D., Autoimmune NZB/NZW F1 mice utilize B-cell receptor editing for generating high affinity anti-dsDNA autoantibodies from low affinity precursors. Eur. J. Immunol. 33:2469-2478, 2003
65. Hoyer, B.F., Moser, K., Hauser, A.E., Peddinghaus, A., Voigt, C., Eilat, D., Radbruch, A., Hiepe, F., and Manz, R.A. Short-lived plasmablasts and long-lived plasma cells contribute to chronic humoral autoimmunity in NZB/NZW mice. J. Exp. Med. 199:1577-1584, 2004
66. Amital, H., Heilweil, M., Ulmansky, R., Szafer, F., Bar-Tana, R., Morel, L., Foster, M.H., Mostoslavsky, G., Eilat, D., Pizov, G., and Naparstek, Y. Treatment with a laminin-derived peptide suppresses lupus nephritis. J. Immunol. 175:5516-5523, 2005
67. Yarkoni, Y., Fischel, R., Kat, I., Yachimovich-Cohen, N., and Eilat, D. Peripheral B cell receptor editing may promote the production of high affinity autoantibodies in CD22-deficient mice. Eur. J. Immunol. 2006, In Press
Competitive Research Grants
65. Israel Academy of Sciences, Basic Research Grants
a. 1995-1997 Molecular cloning and immunological characterization of cell-surface proteins as possible mediators of tissue destruction in autoimmune disease $ 132,000
b. 1998-2001 Molecular and biological properties of a glomerular protein, cross-reactive with pathogenic anti-DNA autoantibodies in SLE $ 151,000
c. 2001-2005 Anti-DNA knock-in mice as models for studying B-cell tolerance mechanisms and the development of autoimmune disease $ 290,000
d. 2005-2009 The role of transitional B cells and peripheral receptor editing in the generation of anti-DNA autoantibodies $ 260,000
66. US-Israel Binational Foundation (BSF)
a. 1995-1997 Expression of autoimmune immunoglobulin genes in transgenic mice
$ 114,000
b. 1998-2001 Mechanism of B cell tolerance and autoimmunity in anti-DNA knock-in mice. $ 150,000
67. German-Israel Foundation (GIF)
a. 1996-1998 Targeting of autoantibody-encoding genes to the mouse genome and its effect on tolerance induction and autoimmune disease. DM 266,000
b. 2000-2003 Analysis of B cell subsets and the germinal center reaction in autoimmune transgenic mice , targeted with anti-DNA heavy chain DM 300,000